CHARACTERIZATION AND QUANTIFICATION OF ACTIVE COMPOUNDS IN PARTHENIUM HYSTEROPHORUS FOR DEVELOPMENT OF A BIO-HERBICIDE TO REDUCE RELIANCE ON SYNTHETIC HERBICIDES

Dr. Bushra Baig
Research Assistant, Department of Environmental Sciences, University of Peshawar, Peshawar 25120, Pakistan, bushi08@yahoo.com
Dr. Saeeda Yousaf
Assistant Professor, Department of Environmental Sciences, University of Peshawar, Peshawar 25120, Pakistan, saeeda@uop.edu.pk
Dr. Javaid Iqbal
Assistant Professor (IPFP), University of Lakki Marwat, 28420, Khyber Pakhtunkhwa, Pakistan. drjavaidenv@gmail.com
Dr. Hina
Research Assistant, Department of Environmental Sciences, University of Peshawar, Peshawar 25120, Pakistan, hinag669@yahoo.com
Dr. Aneeqa Naz
Assistant Professor, Department of Microbiology, Emerson University Multan, 66000, Pakistan aneeqa.naz@eum.edu.pk
Dr. Muhammad Bilal
Research Assistant, Department of Environmental Sciences, University of Peshawar, Peshawar 25120, Pakistan, afridi_online2002@yahoo.com

Abstract

:Biopesticides are more environment-friendly than synthetic insecticides. This research aimed to see whether there is any indication of phytochemical compounds in the extracts of Parthenium hysterophorus by using methanolic extraction and determination of bio-pesticide residues applied on potato and okra crops, grown in a field at the Nuclear Institute for Food and Agriculture (NIFA), Peshawar, Pakistan. Gas Chromatography-Mass Spectrometry (GC-MS) was used to uncover the phytochemical constituents in P. hysterophorus. The outcomes revealed that alkaloids, flavonoids, carbohydrates, proteins, phenols, and tannins were present in the methanolic extracts. Quantitative analyses showed a total of 18 active components in P. hysterophorus dominated by Octadecanoic acid (40%),Carotol (10.5%), and Delta Bisabolol (10.5%).The compounds with lower concentrations were Sabinene (0.32%) and Methhoxy-4-vinyl phenol (0.54%); while, the others with higher concentrations were Propan 1,1 diethoxy (92%), Beta-Caryophyllene (71%), Ledol (84%) and Myristyl chloride (78%).A very simple and rapid QUECHER method was used for sample preparation of okra and potato for the determination of bio-pesticide residues and all the residues were below the Maximum Residual Limit (MRL). The current study reveals that the P. hysterophorus extract was capable of herbicidal potential. The lowest number of whitefly and aphid per leaf was recorded in Parthenium treated plot while the highest number of whitefly and aphid per leaf was recorded in the control plot.

Keywords:

:Bio-pesticides, GC-MS, Parthenium hysterophorus, Phyto chemical screening, toxic residues.


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References


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