DESIGN AND ANALYSIS OF PROTEIN SEPARATION TECHNIQUE IN A MICROFLUIDIC DEVICE

Raghunathareddy M V Dept of ECE, Cambridge Institute of Technology, Bangalore-36 Email: hod.ece@cambridge.edu.in
Indumathi G Dept of ECE, Cambridge Institute of Technology, Bangalore-36

Abstract

Electrophoretic transport across channel stream has been analyzed with different pH concentration of protein molecules. Isoelectric focusing chip model with separating laminar flow region has been introduced in the designed channel. This laminar stream separated the protein molecules with different concentration. Four different protein molecules with different isoelectric point values are considered in the analysis. Protein 1, Protein 2, Protein 3 and Protein 4 has been analyzed for different pressure distribution and velocity magnitude. Maxim pressure distribution of 1296.5×10-5Pa is observed and velocity magnitude of 20×10-5m/s is obtained for designed channel.

Keywords:

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Isoelectric, Microfluidic, Channel, Protein, pH Concentration, laminar flow


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