Beenish Khurshid1*, Saira Farman1, Zahida Parveen1, Sulaiman Shams1, Asma Waheed Qureshi2, Asma Ahmed3
Abstract
:The known essentiality of copper towards various biological processes, its function as a DNA intercalator and the positive response by various cancer cells to copper toxicity widens the horizon for research on Cu-coordinated complexes. This research work focuses on the synthesis and biomimetic activities of eight novel copper (II) complexes. The complexes were synthesized by reacting starting precursor of Cu (II) salt with respective S- and N-donor ligands. The ligands used in this study were thiones and thioamides derivatives. [Cu(phen)(Ln)] type complexes were synthesized by taking (1:1:1) equivalent of all the three reactants in ethanol, where L is S-, N-donor atom (L1= (N-(4-benzoylphenyl)-2-pyridinecarbothioamide,L2=N-(4-morpholinophenyl)-2-pyridinecarbothioamide and L3= N-(2,4,6-Trimethylbenzyl)-2-pyridinecarbothioamide) and phen is 1,10-phenanthroline. Copper (II) complexes of general formula [Cu(L)3] were synthesized by reacting (1:3) of copper(II) salt and above cited S-, N- ligands.
The complexes were characterized by elemental analysis (CHNS), IR and ESI-MS. All the techniques confirmed the proposed structure of synthesized complexes.
In order to study the potential biological activity of the newly synthesized compounds, various assays were performed. The complexes showed moderate antibacterial activity against all the four strains Staphylococcus aureus, Bascillus subtilis (gram positive), Escherichia coli and Shagella (gram negative) at <1.25μg/mL with MIC 0.3125μg/mL against S. Aureus and MIC 0.625μg/mL against Shagella (gram negative). Copper (II) complexes were screened for their antifungal activity by disc diffusion method which showed excellent antifungal activity. Almost all the complexes showed 100% inhibition against Aspergillus niger. The antioxidant activity of these compounds was evaluated by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. The compounds showed 60% to 78% oxidation inhibition. The complex [Cu(I-10 Phen)(Thiomaltol)]2+ showed highest oxidation inhibition.
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