POSTNATAL MORPHOGENSIS OF GASTROINTESTINAL TRACT AND LIVER OF OFFSPRING FROM MOTHERS WITH CHRONIC TOXIC HEPATITIS
Nazarova M.B
Doctor of Medical Sciences, Professor, TMA, St. Forobi, Almazar region, Tashkent, the Republic of Uzbekistan. Email: author.uzb@mail.ru
Adilbekova D.B.
Doctor of Medical Sciences, Associate Professor, TMA, St. Forobi, Almazar region, Tashkent, the Republic of Uzbekistan. Email: dilorom.adilbekova65@gmail.com
Khatamov A.I
Assistant Professor, TMA, St. Forobi, Almazar region, Tashkent, the Republic of Uzbekistan. Email: author.uzb@mail.ru
Sharafutdinov K.K
Assistant Professor, TMA, St. Forobi, Almazar region, Tashkent, the Republic of Uzbekistan. Email: author.uzb@mail.ru
Salaeva Z.Sh
Assistant Professor, TMA, St. Forobi, Almazar region, Tashkent, the Republic of Uzbekistan. Email: author.uzb@mail.ru
Babajanova F.R
Assistant Professor, TMA, St. Forobi, Almazar region, Tashkent, the Republic of Uzbekistan. Email: author.uzb@mail.ru
Abstract
The experiments were carried out on sexually mature white outbred female rats weighing 170-190 g. The model of toxic hepatitis in experimental animals was obtained by weekly administration of heliotrin at the rate of 0.5 mg/100 g of animal mass for 6 weeks. After 10 days from the last injection to females, males were paired. The experimental animals were rat pups aged 3, 7, 14, 21 days old, born and fed by mothers with chronic toxic hepatitis. These study periods are consistent with the generally recognized division of the age periods in rats: the neonatal period (1-5 days), the suckling period (6-21 days). The work was carried out in accordance with the "Rules for work using laboratory animals". The animals were decapitated under Ether anesthesia. The control group consisted of rat pups born and fed by healthy intact females, which were injected with an appropriate amount of isotonic solution instead of heliotrin at the same time.
For the study, pieces were taken from the stomach, small intestine, large intestine and liver of control and experimental animals on the 3rd, 7th and 21st days of postnatal life. The isolated material from the organs was fixed in a 10% solution of neutral formalin and Carnoy's liquid. The resulting material was subjected to morphological, morphometric and electron microscopic research methods. In the course of experimental studies, the effect of chronic liver pathology was revealed in the mother on the postnatal morphogenesis of the organs of the gastrointestinal tract of the offspring. Experiments have shown that toxic maternal hepatitis negatively affects postnatal growth, development and formation and the morphological and functional state of the vascular-tissue structures of the organs of the gastrointestinal tract and liver of the offspring, causing pathomorphological changes in their vascular-tissue structures, which subsequently lead to a lag, delay in the processes of postnatal development and their formation.
All this necessitates the development of scientifically grounded therapeutic and prophylactic measures in relation to children born and fed by mothers with liver pathology, in order to prevent the consequences of development of the disease.
Keywords:
toxic hepatitis, "mother-offspring", gastrointestinal tract, liver, ontogenesis.
References
1. Shubina O.S., Kireeva Y.V. Morphological features of the liver of the offspring of white rats under conditions of lead intoxication. // VII congress international. assoc. morphologists; Morphology. - 2006. - T.129, No. 4. - S. 143. [Smekalina O.U., Bryuhin G.V. Morphofunctional characteristics of endocrine and mast cells of the duodenal mucosa of the offspring of female rats with chronic experimental liver damage. Bulletin of the South Ural State University. 2011; 26 (7): 120-124].
2. Yakovleva L.M., Lyubovtseva L.A. Morphofunctional changes in the ileum of rats after ethanol intoxication. Morphology. 2012; 141 (1): 62-65.
3. Ilinykh M.A., Bryukhin G.V. Structurally functional formation of the pancreas of the offspring of animals with chronic experimental damage to the hepatobiliary system of various origins. Chelyabinsk State Bulletin Pedagogical University. 2006; 4 (6): 113-123.
4. Serysheva O.Yu., Bryukhin G.V. Morphofunctional characteristics of duodenal crypt epithelium in offspring of female rats with experimental liver damage. Morphology. 2013; 144 (4): 36-41.
5. Smekalina O.Y., Bryukhin G.V. Morphofunctional characteristics of endocrine and mast cells of the duodenal mucosa of the offspring of female rats with chronic experimental liver damage. Bulletin of the South Ural State university. 2011; 26 (7): 120-124.
6. Medved V.I., Gritsay I. Liver dysfunctions in pregnant women: Impact on pregnancy, fetal health and labor outcome \\ Health of Ukraine. - 2015 –Special. Issue - S. 24-27
7. Vorobeva V.A. Novopoltseva E. G. Krasilnikova N. E. Features of gastrointestinal tract lesions in premature infants with intrauterine infections and methods of their correction // Questions of modern pediatrics. - 2006; 4: 120-125.
8. Budanov P.V. Strizhakov A.N. Etiology, pathogenesis, diagnosis and treatment of intrauterine infection // Questions of gynecology, obstetrics and perinatology. - 2010; 9 (3): 61-71.
9. Goryacheva L.G. Shilova I.V. The course of chronic hepatitis B in children born to mothers with HB-Virus infection // Children's infections. - 2015.
10. Kowalska-Kanka A. The concentrations of bile acids and erythropoietin in pregnant woman with intrahepatic cholestasis and the state of the fetus and newborn // Medycyna Wieku Rozwojowego. – 2013; 17(3): 103-109.
11. Bonney. J.H. Kwa M.E.-Aryee. Fatal hepatitis E viral infections in pregnant in woman in Ghana: a case series. // BMC Res Notes – 2012; 5: 478.
12. Knight L., Todd G., Stevens Johnson. Syndrome and Toxic Epidermal Necrolysis: Maternal and Foetal Outcomes in Twenty-Two Consecutive Pregnant HIV Infected Women // Plos One. 2015; 10(8): Р. e0135501.
13. Chełchowska M, Gajewska J., Ambroszkiewicz J., Laskowska-Klita T. Exposition of pregnant women and their children on toxic effect of lead from tobacco smoke// Przeglad Lekarski. 2009; 66(10): 869-72.
14. Yin Y, Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong Province, China.//Plos One.- 2017; 12(6): Р. e0178671
15. Chen Zhao Z. Course-, dose-, and stage-dependent toxic effects of prenatal dexamethasone exposure on fetal articular cartilage development.//Toxicology Letters. 2018; 286: 1-9.
16. Mirderikvand N, Mohammadzadeh Asl B. Embryo toxic effects of depleted uranium on the morphology of the mouse fetus.//Iranian Journal Of Pharmaceutical Research. 2014; 13(1): 199-206.
17. Zhu Y, Pang Y. Association Between Chronic Exposure to Tobacco Smoke and Accumulation of Toxic Metals in Hair Among Pregnant Women.//Biological Trace Element Research. 2018 Mar 01.
18. Zhao Y. Zhang X. Viral hepatitis vaccination during pregnancy.//Human Vaccines & Immunotherapeutics . 2016; 12(4): 894-902.
19. Page C.M. Hughes B.L. Hepatitis C in Pregnancy: Review of Current Knowledge and Updated Recommendations for Management// Obstet Gynecol Surv. 2017; 72(6): 347-355.
20. Schanche M, Avershina E, Dotterud C. High-Resolution Analyses of Overlap in the Microbiota Between Mothers and Their Children.//Current Microbiology [Curr Microbiol] 2015 Aug; 71(2): 283-90.
21. Ban L., Gibson J.E., West J. Association between perinatal depression in mothers and the risk of childhood infections in offspring: a population-based cohort study.//BMC Public Health. 2010; 31(10): 799.
22. Avershina E; Storrø O. Johnsen R. Major faecal microbiota shifts in composition and diversity with age in a geographically restricted cohort of mothers and their children.// FEMS Microbiology Ecology. 2014 Jan; 87(1): 280-90.
23. Gurnee E.A., Ndao I.M; Gut Colonization of Healthy Children and Their Mothers With Pathogenic Ciprofloxacin-Resistant Escherichia coli.//The Journal Of Infectious Diseases. 2015; Dec 15; 212(12): 1862-8.
24. Lu L.L, Chen B.X. Maternal transmission risk and antibody levels against hepatitis B virus e antigen in pregnant women.//Int J Infect Dis] 2014 Nov; 28: 41-4.
25. Julsgaard M. Christensen L.A; Concentrations of Adalimumab and Infliximab in Mothers and Newborns, and Effects on Infection.//Gastroenterology. 2016 Jul; 151(1): 110-9.
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