EVALUATION OF THE BIOLOGICAL ACTIVITY OF PHYLLALBIN AND RUTIN, AND APPLICATION IN WOUND DRESSINGS

Natalya Y. Tseomashko MD, PhD, Head of the Department of Medical and Genetic Research of the Interuniversity Research Laboratory of the Tashkent Medical Academy of the Ministry of Health of the Republic of Uzbekistan. Tashkent, Uzbekistan E-mail: tsne_77@list.ru
Aziza Dj. Abidova Junior Researcher, Interuniversity Research Laboratory Interuniversity Research Laboratory of the Tashkent Medical Academy of the Ministry of Health of the Republic of Uzbekistan. Tashkent, Uzbekistan E-mail: info@tma.uz
Sardor Dj. Makhmudov Basic doctoral student of the Institute of Bioorganic Chemistry of the Academy of Sciences of the Republic of Uzbekistan. Tashkent, Uzbekistan E-mail: info@tma.uz
Shahnoz S. Azimova Doctor of Science, prof. Institute of the Chemistry of Plant Substances Academy of Sciences at Uzbekistan. Tashkent, Uzbekistan. E-mail: info@tma.uz
Salima F. Aripova

Abstract

To accelerate the healing of wounds, ulcers, burns polyfunctional wound dressings (WD) are required. The functional properties of WD can be expanded by introducing in situ or ex situ to the structure of their biologically active components (BAC) composition. In our studies, we propose to use as a BAC the alkaloid phillalbine (Ph) which is isolated from plants of the genus Convolvulus, flavonoid quercetin (Qu) isolated from buds of Saphora japonica and glycoside of quercetin flavonoid - rutin (Ru). It is found that Ph can be used as skin fibroblast cells proliferator. Ph at a concentration of 12.5-25 μg / ml increases fibroblast proliferation by 25-79%. Ru at a concentration of 25 μg / ml exhibits antimicrobial activity against a wide range of microorganisms. Qu is a well-known antioxidant. BAC data is injected ex situ into collagen (CC) gel. Compositions of polyfunctional WD in membrane form were created: СC-Qu-Ru-Ph and СC-Qu-Ru, and were studied in comparison with commercial preparations "NeuSkin-F" (Eucare Pharmaceuticals (P) Limited, India) on experimental models of burn wounds. It was found that CC-Qu-Ru-Ph possesses high congruence and adhesion to the surface of the wound bed and accelerates wound healing 1.49 times faster compared to untreated burn wounds of the same size, and 1.27 times faster than "NeuSkin-F". CC-Qu-Ru-Ph and CC-Qu-Ru are non-toxic, do not have a local irritant effect, and are histocompatible. The safety of the obtained WD was confirmed by histomorphological studies of organs and tissues of rats, who took high doses of WD compositions (5000 mg / kg) orally for a month.

Keywords:

phillalbin, quercetin, rutin, collagen, proliferator, antioxidant, wound dressing


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