COMPARATIVE EVALUATION OF PHYTOCHEMICALS, ANTIOXIDANT AND ANTIBACTERIAL EFFICIENCY OF CASSIA OCCIDENTALIS AND PITHECELLOBIUM DULCE LEAVES EXTRACT

B. Rajarajeswari Lecturer, Department of Botany, DKW College, Nellore-524003, Andhra Pradesh, India.
B. Praveen Kumar Biology Faculty, Department of biology, Master JEE IIT academy,Kalpakkam,Tamil Nadu, India.
A.Amruthavalli Associate Professor, Department of Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India. audipudiamrita@gmail.com

Abstract

In the present study, two indigenous plant species of C.occidentalis and P. dulce are selected and comparative analysis of phytochemicals, antioxidant and anti-bacterial activity of leaves extracts was carried out with different solvents of aqueous, petroleum ether, chloroform and ethanol. The extractive values of these extracts ranged from 3.9-17.5 %w/w and showed the existence of alkaloids, flavonoids, sterols, anthraquione, cardiac glycosides, tannins and terpenoids of both the plant species. The total phenolic content of both the plant species were in range of 10- 92 µg/mg and the highest was recorded 92.45 ±1.26 µg/mg in ethanolic extract of C.occidentalis and the least value10.81 ± 0.31 µg/mg was observed in the petroleum extract of the P.dulce. The TFC are in the range of 9-148 µg/mg in both plants. The highest value of 148.99 ± 6.01µg/mg was observed in ethanolic extract of C.occidentalis and 79.49± 1.59µg/mg was recorded in petroleum ether of P.dulce respectively. The ethanolic and chloroform extracts showed strong antioxidant and reducing power potentials. Ethanolic and chloroform extracts given the best results in anti-bacterial screening and the MIC were in the range of 200-800µg/ml confirming the pharmacognostic feature.

Keywords:

Phytochemical, antioxidant, alkaloids, ethanolic extracts, antibacterial.


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