C. Rajasekaran, Sk. Roshakhi Sultana, N. J. Abineshwar, Rachitha Radhakrishnan
Abstract
Aims and objectives: This prospective study is aimed to evaluate the effect of vinegar (1% Acetic acid) in treatment of
wounds infected with Pseudomonas.
Methods: A total of 60 patients attending the OPD of Vinayaka Mission’s Kirupananda Variyar Medical College with any
wound and a positive culture report for Pseudomonas aeruginosa was included in our study after obtaining proper consent.
The following patients are randomised into two groups of 30. Group A (Test) was subjected to vinegar dressing (1% acetic
acid) once daily and Group B (Control) was subjected to normal saline dressing. Both groups did not receive enteral or
parenteral antibiotics throughout the period of study. Patients were followed up with wound cultures repeated on day 3,
7, and 14.
Results: The duration of treatment required to eliminate the Pseudomonas from the wounds in the acetic acid group was on
an average 7days less than that required by the saline group. P value was 0⋅001. This was a very significant factor.
Majority of the cultures tested negative after 7 days of treatment with acetic acid as compared to saline dressing
(p0.001) which is also statistically significant. The wounds also showed a marked reduction in wound size.
Conclusion: Vinegar (1%acetic acid) is a holy grail in the treatment of chronic wounds with P. Aeruginosa as it is
highly efficacious and a cost-effective alternative.
Keywords:
:Pseudomonas aeruginosa, 1% Acetic acid, Vinegar dressing, Saline dressing
References
1. McGregor AD, McGregor I. Free skin grafts. In: McGregor AD, McGregor I, editors. Fundamental techniques of plastic
surgery, 10th edn. Philadelphia, PA: Churchill Livingstone, 2000:35 – 59.
2. Edwards-Jones V, Greenwood JE. What’s new in burn microbiology? James Laing Memorial Prize Essay 2000. Burns
2003;29:15–24.
3. Bitsch M, Saunte DM, Lohmann M, Holstein PE, Jorgensen B, Gottrup F. Standardised method of surgical treatment of
chronic leg ulcers. Scand J Plast Reconstr Surg Hand Surg 2005;39:162–9.
4. Gruber RP, Vistnes L, Pardoe R. The effect of commonly used antiseptics on wound healing. Plast Reconstr Surg
1975;55:472–6.
5. Lineaweaver W, Howard R, Soucy D, McMorris S, Freeman J, Crain C, Robertson J, Rumley T. Topical antimicrobial
toxicity. Arch Surg 1985;120:267 – 70.
6. O’Meara S, Cullum N, Majid M, Sheldon T. Systematic reviews of wound care management: (3) antimicrobial agents for
chronic wounds; (4) diabetic foot ulceration. Health Technol Assess 2000;4:1-237.
7. Stewart CM, Cole MB, Legan JD, Slade L, Vandeven MH, Schaffner DW, et al. Staphylococcus aureus growth boundaries:
Moving towards mechanistic predictive models based on solute-specific effects. Appl Environ Microbiol 2002;68:1864-71.
8. Thomas LV, Wimpenny JW, Davis JG. Effect of three preservatives on the growth of Bacillus cereus, vero cytotoxigenic
Escherichia coli and Staphylococcus aureus, on plates with gradients of pH and sodium chloride concentration. Int J Food
Microbiol 1993;17:289-301.
9. Hunt TK, Twomey P, Zederfeldt B, Dunphy JE. Respiratory gas tensions and pH in healing wounds. Am J Surg
1967;114:302-7. 14. Hunt TK, Beckert S. Therapeutical and practical aspects of oxygen in wound healing. In: Lee B,
editor. The Wound Management
10. Manual. New York: McGraw-Hill Professional; 2004. p. 44-54.
11. Salami AA, Imosemi IO, Owoeye OO. Comparación del efecto de Clorhexidina, agua corriente y suero salino en
curación de heridas. Int J Morphol 2006;24:673–6.
12. Mustoe T. Understanding chronic wounds: a unifying hypothesis on their pathogenesis and implications for therapy. Am
J Surg 2004;187(5 Suppl):65S – 70.
13. Thomas GW, Rael LT, Bar-Or R, Shimonkevitz R, Mains CW, Slone DS, Craun ML, Bar-Or D. Mechanisms of delayed wound
healing by commonly used antiseptics. J Trauma 2009;66:82–91.
14. Price-Whelan A, Dietrich LEP, Newman DK. Pyocyanin alters redox homeostasis and carbon flux through central
metabolic pathways in Pseudomonas aeruginosa PA14. J Bacteriol 2007;189:6372 – 81.
15. Costerton W, Veeh R, Shirtliff M, Pasmore M, Post C, Ehrlich G. The application of biofilm science to the study and
control of chronic bacterial infections. J Clin Invest 2003;112:1466–77.
16. Vuong C, Kocianova S, Voyich JM, Yao Y, Fischer ER, DeLeo FR, Otto M. A crucial role for exopolysaccharide
modification in bacterial biofilm formation, immune evasion, and virulence. J Biol Chem 2004;279:54881–6.
17. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a com- mon cause of persistent infections. Science
1999;284:1318–22.
18. Costerton W, Veeh R, Shirtliff M, Pasmore M, Post C, Ehrlich G. The application of biofilm science to the study and
control of chronic bacterial infections. J Clin Invest 2003;112:1466–77.
19. Unal S, Ersoz G, Demirkan F, Arslan E, Tütüncü N, Sari A. Analysis of skin-graft loss due to infection:
infection-related graft loss. Ann Plast Surg 2005;55:102 – 6.
20. Thompson LJ, Merrell DS, Neilan BA, Mitchell H, Lee A, Falkow S. Gene expression profiling of Helicobacter pylori
reveals a growth-phase-dependent switch in virulence gene expression. Infect Immun 2003;71:2643–55.